Reticulophagy regulator 1 (Retreg1), recombinant rat

Organism: Rattus norvegicus (Rat). Source: in vitro E.coli expression system. Expression Region: 1-480aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MASPAPEEHA TQGCPATEEQ EPRPGVPGEE AGPEGAGPQV EEAAGRVAAA LTWLLGEPVL WLGWRADELL SWKRPLRSLL AFLGANLLFW FLALTPWRVY HLISVMILGR VIMQIIKDMV LSRARGAQLW RSLSESWEVI NSKPDERPRL SHCIAESWMN FSIFLQEMSL FKQQSPGKFC LLVCSVCTFF TILGSYIPGV ILSYLLLLFA FLCPLFKCND IGQKIYSKVK SILLKLDFGI GEYINQKKRE RSEADKEKSH KDDSEIDFSA LCPKISLTVA AKELSVSDTD VSEVSWTDNG TFNLSEGYTP QTDTSDDLDR PSEEVFSRDL SDFPSLENGA GTNDEDELSL GLPTELKTKK QQLNSAHRPS KDRQSAAGLS LPLKSDQALH LMSNLAGDVI TAAMTAAIKD QLEGARQALA QAAPTPGDDT DTEEGDDFEL LDQAELDQIE SELGLTQDQG AEAQQSKKSS GFLSNLLGGH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Endoplasmic reticulum-anchored autophagy receptor that mediates ER delivery into lysosomes through sequestration into autophagosomes. Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins. Required for long-term survival of nociceptive and autonomic ganglion neurons. Reference: n/a. Function: nan