Prolyl 4-hydroxylase subunit alpha-1 (P4HA1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 18-534aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: HPGFFTSIGQ MTDLIHTEKD LVTSLKDYIK AEEDKLEQIK KWAEKLDRLT STATKDPEGF VGHPVNAFKL MKRLNTEWSE LENLVLKDMS DGFISNLTIQ RQYFPNDEDQ VGAAKALLRL QDTYNLDTDT ISKGNLPGVK HKSFLTAEDC FELGKVAYTE ADYYHTELWM EQALRQLDEG EISTIDKVSV LDYLSYAVYQ QGDLDKALLL TKKLLELDPE HQRANGNLKY FEYIMAKEKD VNKSASDDQS DQKTTPKKKG VAVDYLPERQ KYEMLCRGEG IKMTPRRQKK LFCRYHDGNR NPKFILAPAK QEDEWDKPRI IRFHDIISDA EIEIVKDLAK PRLRRATISN PITGDLETVH YRISKSAWLS GYENPVVSRI NMRIQDLTGL DVSTAEELQV ANYGVGGQYE PHFDFARKDE PDAFKELGTG NRIATWLFYM SDVSAGGATV FPEVGASVWP KKGTAVFWYN LFASGEGDYS TRHAACPVLV GNKWVSNKWL HERGQEFRRP CTLSELE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Reference: "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009). Function: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.