Nampt, Recombinant, Mouse (Visfatin, PBEF, Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamid

Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Source: Recombinant protein corresponding to aa1-491 of mouse Nampt, fused to FLAG-tag at N-terminal, expressed in CHO cells. Molecular Weight: ~55kD, Endotoxin: <0.01EU/ug (LAL), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.