Long-chain-fatty-acid--CoA ligase 5 (ACSL5), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 33-683aa. Protein Length: Cytoplasmic Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: TRPQPVLPLL DLNNQSVGIE GGARKGVSQK NNDLTSCCFS DAKTMYEVFQ RGLAVSDNGP CLGYRKPNQP YRWLSYKQVS DRAEYLGSCL LHKGYKSSPD QFVGIFAQNR PEWIISELAC YTYSMVAVPL YDTLGPEAIV HIVNKADIAM VICDTPQKAL VLIGNVEKGF TPSLKVIILM DPFDDDLKQR GEKSGIEILS LYDAENLGKE HFRKPVPPSP EDLSVICFTS GTTGDPKGAM ITHQNIVSNA AAFLKCVEHA YEPTPDDVAI SYLPLAHMFE RIVQAVVYSC GARVGFFQGD IRLLADDMKT LKPTLFPAVP RLLNRIYDKV QNEAKTPLKK FLLKLAVSSK FKELQKGIIR HDSFWDKLIF AKIQDSLGGR VRVIVTGAAP MSTSVMTFFR AAMGCQVYEA YGQTECTGGC TFTLPGDWTS GHVGVPLACN YVKLEDVADM NYFTVNNEGE VCIKGTNVFK GYLKDPEKTQ EALDSDGWLH TGDIGRWLPN GTLKIIDRKK NIFKLAQGEY IAPEKIENIY NRSQPVLQIF VHGESLRSSL VGVVVPDTDV LPSFAAKLGV KGSFEELCQN QVVREAILED LQKIGKESGL KTFEQVKAIF LHPEPFSIEN GLLTPTLKAK RGELSKYFRT QIDSLYEHIQ D. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells. Reference: "Regulation of enterocyte apoptosis by acyl-CoA synthetase 5 splicing."Gassler N., Roth W., Funke B., Schneider A., Herzog F., Ehemann V., Sykora J., Haas T.L., Walczak H., Ganten T., Zentgraf H., Erb P., Alonso A., Autschbach F., Schirmacher P., Knuechel R., Kopitz J. Gastroenterology 133:587-598(2007). Function: Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.