Glycogen phosphorylase, liver form (PYGL),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-846aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: AKPLTDQEKR RQISIRGIVG VENVAELKKS FNRHLHFTLV KDRNVATTRD YYFALAHTVR DHLVGRWIRT QQHYYDKCPK RVYYLSLEFY MGRTLQNTMI NLGLQNACDE AIYQLGLDIE ELEEIEEDAG LGNGGLGRLA ACFLDSMATL GLAAYGYGIR YEYGIFNQKI RDGWQVEEAD DWLRYGNPWE KSRPEFMLPV HFYGKVEHTN TGTKWIDTQV VLALPYDTPV PGYMNNTVNT MRLWSARAPN DFNLRDFNVG DYIQAVLDRN LAENISRVLY PNDNFFEGKE LRLKQEYFVV AATLQDIIRR FKASKFGSTR GAGTVFDAFP DQVAIQLNDT HPALAIPELM RIFVDIEKLP WSKAWELTQK TFAYTNHTVL PEALERWPVD LVEKLLPRHL EIIYEINQKH LDRIVALFPK DVDRLRRMSL IEEEGSKRIN MAHLCIVGSH AVNGVAKIHS DIVKTKVFKD FSELEPDKFQ NKTNGITPRR WLLLCNPGLA ELIAEKIGED YVKDLSQLTK LHSFLGDDVF LRELAKVKQE NKLKFSQFLE TEYKVKINPS SMFDVQVKRI HEYKRQLLNC LHVITMYNRI KKDPKKLFVP RTVIIGGKAA PGYHMAKMII KLITSVADVV NNDPMVGSKL KVIFLENYRV SLAEKVIPAT DLSEQISTAG TEASGTGNMK FMLNGALTIG TMDGANVEMA EEAGEENLFI FGMRIDDVAA LDKKGYEAKE YYEALPELKL VIDQIDNGFF SPKQPDLFKD IINMLFYHDR FKVFADYEAY VKCQDKVSQL YMNPKAWNTM VLKNIAASGK FSSDRTIKEY AQNIWNVEPS DLKISLSNES NKVNG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Reference: The DNA sequence and analysis of human chromosome 14.Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.Nature 421:601-607(2003). Function: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.