Glycogen phosphorylase, brain form (PYGB) (R61A,K192A), recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-843aa(R61A,K192A). Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AKPLTDSEKR KQISVRGLAG LGDVAEVRKS FNRHLHFTLV KDRNVATPRD YFFALAHTVA DHLVGRWIRT QQHYYERDPK RIYYLSLEFY MGRTLQNTMV NLGLQNACDE AIYQLGLDLE ELEEIEEDAG LGNGGLGRLA ACFLDSMATL GLAAYGYGIR YEFGIFNQKI VNGWQVEEAD DWLRYGNPWE AARPEYMLPV HFYGRVEHTP DGVKWLDTQV VLAMPYDTPV PGYKNNTVNT MRLWSAKAPN DFKLQDFNVG DYIEAVLDRN LAENISRVLY PNDNFFEGKE LRLKQEYFVV AATLQDIIRR FKSSKFGCRD PVRTCFETFP DKVAIQLNDT HPALSIPELM RILVDVEKVD WDKAWEITKK TCAYTNHTVL PEALERWPVS MFEKLLPRHL EIIYAINQRH LDHVAALFPG DVDRLRRMSV IEEGDCKRIN MAHLCVIGSH AVNGVARIHS EIVKQSVFKD FYELEPEKFQ NKTNGITPRR WLLLCNPGLA DTIVEKIGEE FLTDLSQLKK LLPLVSDEVF IRDVAKVKQE NKLKFSAFLE KEYKVKINPS SMFDVHVKRI HEYKRQLLNC LHVVTLYNRI KRDPAKAFVP RTVMIGGKAA PGYHMAKLII KLVTSIGDVV NHDPVVGDRL KVIFLENYRV SLAEKVIPAA DLSQQISTAG TEASGTGNMK FMLNGALTIG TMDGANVEMA EEAGAENLFI FGLRVEDVEA LDRKGYNARE YYDHLPELKQ AVDQISSGFF SPKEPDCFKD IVNMLMHHDR FKVFADYEAY MQCQAQVDQL YRNPKEWTKK VIRNIACSGK FSSDRTITEY AREIWGVEPS DLQIPPPNIP RD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Reference: n/a. Function: nan