Galectin-3, active (LGALS3), Recombinant, Human

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. {ECO:0000250, ECO:0000269, PubMed:15181153, ECO:0000269, PubMed:19594635, ECO:0000269, PubMed:19616076}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by its ability to agglutinate human red blood cells is less than 10ug/ml. Sequence: ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI, Molecular Weight: 26.0kD, PubMed ID: 2261464, 2402511, 2022338, 2009535, 7682704, 9439577, 14702039, 12508121, 15489334, 8253806, 9501082, 15181153, 19594635, 19497882, 19616076, 21269460, 9582341, Gene Name: LGALS3, MAC2, Swiss Prot: P17931, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.