Extracellular calcium-sensing receptor (CASR), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 20-612aa. Protein Length: Extracellular Domain. Tag Info: N-terminal GST-tagged. Target Protein Sequence: YGPDQRAQKK GDIILGGLFP IHFGVAAKDQ DLKSRPESVE CIRYNFRGFR WLQAMIFAIE EINSSPALLP NLTLGYRIFD TCNTVSKALE ATLSFVAQNK IDSLNLDEFC NCSEHIPSTI AVVGATGSGV STAVANLLGL FYIPQVSYAS SSRLLSNKNQ FKSFLRTIPN DEHQATAMAD IIEYFRWNWV GTIAADDDYG RPGIEKFREE AEERDICIDF SELISQYSDE EEIQHVVEVI QNSTAKVIVV FSSGPDLEPL IKEIVRRNIT GKIWLASEAW ASSSLIAMPQ YFHVVGGTIG FALKAGQIPG FREFLKKVHP RKSVHNGFAK EFWEETFNCH LQEGAKGPLP VDTFLRGHEE SGDRFSNSST AFRPLCTGDE NISSVETPYI DYTHLRISYN VYLAVYSIAH ALQDIYTCLP GRGLFTNGSC ADIKKVEAWQ VLKHLRHLNF TNNMGEQVTF DECGDLVGNY SIINWHLSPE DGSIVFKEVG YYNVYAKKGE RLFINEEKIL WSGFSREVPF SNCSRDCLAG TRKGIIEGEP TCCFECVECP DGEYSDETDA SACNKCPDDF WSNENHTSCI AKEIEFLSWT EPF. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Senses changes in the Extracellular domain concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger syst. Reference: Rab1 small GTP-binding protein regulates cell surface trafficking of the human calcium-sensing receptor.Zhuang X., Adipietro K.A., Datta S., Northup J.K., Ray K.Endocrinology 151:5114-5123(2010). Function: G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis