D-3-phosphoglycerate dehydrogenase (PHGDH), partial, recombinant human

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-251aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-GST-tagged. Target Protein Sequence: AFANLRKVLI SDSLDPCCRK ILQDGGLQVV EKQNLSKEEL IAELQDCEGL IVRSATKVTA DVINAAEKLQ VVGRAGTGVD NVDLEAATRK GILVMNTPNG NSLSAAELTC GMIMCLARQI PQATASMKDG KWERKKFMGT ELNGKTLGIL GLGRIGREVA TRMQSFGMKT IGYDPIISPE VSASFGVQQL PLEEIWPLCD FITVHTPLLP STTGLLNDNT FAQCKKGVRV VNCARGGIVD EGALLRALQS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate. Reference: n/a. Function: nan