Citrullinated Histone H3 (human recombinant)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay17926-100 100 µg -

3 - 10 business days*

560.00€
 
A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice... more
Product information "Citrullinated Histone H3 (human recombinant)"
A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core. Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription. Citrullination is another important post-translational modification that is catalyzed by peptidylarginine deiminase 4 (PAD4) and PAD2. Hypercitrullination of histones leads to the development of neutrophil extracellular traps (NETs), which are a part of the inflammatory response. Neutrophils release these NETs to trap and eradicate bacteria and fungi. Failure to clear citrullinated proteins and NET components following inflammation can result in the production of autoantibodies and anti-citrullinated protein antibodies. The persistence of these antibodies and citrullinated proteins is associated with a number of human diseases including rheumatoid arthritis, systemic lupus erythematosus, Alzheimer's disease, and multiple sclerosis. This product contains purified Histone H3 (human, recombinant) (Cay-10263) that has been modified with PAD4 enzyme, which is removed by affinity chromatography after the reaction. Synonyms: H3, H3C1, Histone H3.1. Purity: >95% estimated by SDS-PAGE. Protein Identity Confirmed by Mass Spec: Yes. Source: Recombinant protein expressed in E. coli, citrullinated with recombinant human PAD4. MW: 15.5 kDa. Formulation: A solution in PBS, pH 7.4. Applications: Immunoreactive with anti-citrulline antibodies by Western blot and ELISA. UniProt Accession #: P68431.
Keywords: H3FA, H3FL, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, Histone H3.1, Histone H3/a, Histone H3/b, Histo
Supplier: Cayman Chemical
Supplier-Nr: 17926

Properties

Application: ELISA, WB
Conjugate: No
Expressed in: E.coli
Origin: Human
MW: 15.5 kD
Purity: >95% estimated by SDS-PAGE
Format: Solution

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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