Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN (Wrn), partial, recombinant mouse

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 482-1207aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-ZZ-tagged. Target Protein Sequence: IKEEQEEEDH LLPEPNAKQI NCLKTYFGHS SFKPVQWKVI HSVLEERRDN VVVMATGYGK SLCFQYPPVY TGKIGIVISP LISLMEDQVL QLELSNVPAC LLGSAQSKNI LGDVKLGKYR VIYITPEFCS GNLDLLQQLD SSIGITLIAV DEAHCISEWG HDFRSSFRML GSLKTALPLV PVIALSATAS SSIREDIISC LNLKDPQITC TGFDRPNLYL EVGRKTGNIL QDLKPFLVRK ASSAWEFEGP TIIYCPSRKM TEQVTAELGK LNLACRTYHA GMKISERKDV HHRFLRDEIQ CVVATVAFGM GINKADIRKV IHYGAPKEME SYYQEIGRAG RDGLQSSCHL LWAPADFNTS RNLLIEIHDE KFRLYKLKMM VKMEKYLHSS QCRRRIILSH FEDKCLQKAS LDIMGTEKCC DNCRPRLNHC LTANNSEDAS QDFGPQAFQL LSAVDILQEK FGIGIPILFL RGSNSQRLPD KYRGHRLFGA GKEQAESWWK TLSHHLIAEG FLVEVPKENK YIKTCSLTKK GRKWLGEASS QSPPSLLLQA NEEMFPRKVL LPSSNPVSPE TTQHSSNQNP AGLTTKQSNL ERTHSYKVPE KVSSGTNIPK KSAVMPSPGT SSSPLEPAIS AQELDARTGL YARLVEARQK HANKMDVPPA ILATNKVLLD MAKMRPTTVE NMKQIDGVSE GKAALLAPLL EVIKHFCQVT SVQTDLLSSA KPHKEQ. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Multifunctional enzyme that has magnesium and ATP-dependent 3'-5' DNA-helicase activity . Has 3'->5' exonuclease activity on forked dsDNA). Reference: n/a. Function: nan