Annexin A1 (Anxa1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 2-346aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: AMVSEFLKQA RFLENQEQEY VQAVKSYKGG PGSAVSPYPS FNVSSDVAAL HKAIMVKGVD EATIIDILTK RTNAQRQQIK AAYLQENGKP LDEVLRKALT GHLEEVVLAM LKTPAQFDAD ELRGAMKGLG TDEDTLIEIL TTRSNEQIRE INRVYREELK RDLAKDITSD TSGDFRKALL ALAKGDRCQD LSVNQDLADT DARALYEAGE RRKGTDVNVF TTILTSRSFP HLRRVFQNYG KYSQHDMNKA LDLELKGDIE KCLTTIVKCA TSTPAFFAEK LYEAMKGAGT RHKALIRIMV SRSEIDMNEI KVFYQKKYGI SLCQAILDET KGDYEKILVA LCGGN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium/phospholipid-binding protein which promotes mbrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It ses to bind from two to four calcium ions with high affinity. Reference: SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.Mol. Cell 50:919-930(2013). Function: Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity