ACSL1 (human, recombinant, aa 48-698)

Acyl-CoA synthetase long-chain family member 1 (ACSL1) is a long-chain acyl-CoA synthase that converts both saturated and unsaturated fatty acids into fatty acyl-CoA esters. It is expressed in liver, heart, adipose tissue, and muscle and, in the presence of TANK-binding kinase 1 (TBK1), localizes to the mitochondria where it promotes fatty acid beta-oxidation. In the absence of TBK1, ACSL1 is localized to the endoplasmic reticulum (ER) and facilitates lipid accumulation. ACSL1 has broad substrate specificity for 16- and 18-carbon saturated fatty acids and 16-20-carbon unsaturated fatty acids. ACSL1 knockdown suppresses palmitate-induced increases in the inflammatory markers TNF-alpha, IL-1beta, and CD11c and inhibits lipid accumulation and the transition into foam cells in THP-1 macrophages, as well as inhibits ferroptosis induced by alpha-eleostearic acid (9(Z),11(E),13(E)-octadecatrienoic acid (Cay-10008349) in BT-549 cells. Levels of ACSL1 are increased in liver, breast, ovarian, and colorectal cancers compared with non-cancerous tissues. Cayman's ACSL1 (human, recombinant, aa 48-698) protein can be used for ELISA, enzyme activity, and Western blot (WB) applications.Synonyms: Acyl-CoA Synthetase Long-chain Family Member 1, FACL1, Fatty Acid CoA Ligase 1, LACS1, Long-chain Fatty Acid-CoA Ligase 1, Palmitoyl-CoA Ligase 1. Purity: >90% estimated by SDS-PAGE. Source: Active recombinant human N-terminal His-tagged ACSL1 expressed in E. coli. Amino Acids: 48-698. MW: 74.62. Formulation: (Request formulation change), 50 mM Tris, pH 8.0, with 150 mM sodium chloride and 10% glycerol. UniProt Accession #: P33121.