5-Hydroxytryptamine receptor 3E (HTR3E), partial, human, recombinant

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 72-229aa & 241-456aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MSAILDVNEQ LHLLSSFLWL EMVWDNPFIS WNPEECEGIT KMSMAAKNLW LPDIFIIELM DVDKTPKGLT AYVSNEGRIR YKKPMKVDSI CNLDIFYFPF DQQNCTLTFS SFLYTVDSML LDMEKEVWEI TDASRNILQT HGEWELLGLS KATAKLSRVA IRRRPSLYVI NLLVPSGFLV AIDALSFYLP VKSGNRVPFK ITLLLGYNVF LLMMSDLLPT SGTPLIGVYF ALCLSLMVGS LLETIFITHL LHVATTQPPP LPRWLHSLLL HCNSPGRCCP TAPQKENKGP GLTPTHLPGV KEPEVSAGQM PGPAEAELTG GSEWTRAQRE HEAQKQHSVE LWLQFSHAMD AMLFRLYLLF MASSIITVIC LWNT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel. Reference: "Characterization of the novel human serotonin receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E."Niesler B., Walstab J., Combrink S., Moeller D., Kapeller J., Rietdorf J., Boenisch H., Goethert M., Rappold G., Bruess M.Mol. Pharmacol. 72:8-17(2007). Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.