LRRC8A (Vector pUC, Accession No. BC051322)

Length: 2433 Sequence: atgattccgg tgacagagct ccgctacttt gcggacacgc agccagcata ccggatcctg aagccgtggt gggatgtgtt cacagactac atctctatcg tcatgctgat gattgccgtc ttcgggggga cgctgcaggt cacccaagac aagatgatct gcctgccttg taagtgggtc accaaggact cctgcaatga ttcgttccgg ggctgggcag cccctggccc ggagcccacc taccccaact ccaccattct gccgacccct gacacgggcc ccacaggcat caagtatgac ctggaccggc accagtacaa ctacgtggac gctgtgtgct atgagaaccg actgcactgg tttgccaagt acttccccta cctggtgctt ctgcacacgc tcatcttcct ggcctgcagc aacttctggt tcaaattccc gcgcaccagc tcgaagctgg agcactttgt gtctatcctg ctgaagtgct tcgactcgcc ctggaccacg agggccctgt cggagacagt ggtggaggag agcgacccca agccggcctt cagcaagatg aatgggtcca tggacaaaaa gtcatcgacc gtcagtgagg acgtggaggc caccgtgccc atgctgcagc ggaccaagtc acggatcgag cagggtatcg tggaccgctc agagacgggc gtgctggaca agaaggaggg ggagcaagcc aaggcgctgt ttgagaaggt gaagaagttc cggacccatg tggaggaggg ggacattgtg taccgcctct acatgcggca gaccatcatc aaggtgatca agttcatcct catcatctgc tacaccgtct actacgtgca caacatcaag ttcgacgtgg actgcaccgt ggacattgag agcctgacgg gctaccgcac ctaccgctgt gcccaccccc tggccacact cttcaagatc ctggcgtcct tctacatcag cctagtcatc ttctacggcc tcatctgcat gtatacactg tggtggatgc tacggcgctc cctcaagaag tactcgtttg agtcgatccg tgaggagagc agctacagcg acatccccga cgtcaagaac gacttcgcct tcatgctgca cctcattgac caatacgacc cgctctactc caagcgcttc gccgtcttcc tgtcggaggt gagtgagaac aagctgcggc agctgaacct caacaacgag tggacgctgg acaagctccg gcagcggctc accaagaacg cgcaggacaa gctggagctg cacctgttca tgctcagtgg catccctgac actgtgtttg acctggtgga gctggaggtc ctcaagctgg agctgatccc cgacgtgacc atcccgccca gcattgccca gctcacgggc ctcaaggagc tgtggctcta ccacacagcg gccaagattg aagcgcccgc gctggccttc ctgcgcgaga acctgcgggc gctgcacatc aagttcaccg acatcaagga gatcccgctg tggatctata gcctgaagac actggaggag ctgcacctga cgggcaacct gagcgcggag aacaaccgct acatcgtcat cgacgggctg cgggagctca aacgcctcaa ggtgctgcgg ctcaagagca acctaagcaa gctgccacag gtggtcacag atgtgggcgt gcacctgcag aagctgtcca tcaacaatga gggcaccaag ctcatcgtcc tcaacagcct caagaagatg gcgaacctga ctgagctgga gctgatccgc tgtgacctgg agcgcatccc ccactccatc ttcagcctcc acaacctgca ggagattgac ctcaaggaca acaacctcaa gaccatcgag gagatcatca gcttccagca cctgcaccgc ctcacctgcc ttaagctgtg gtacaaccac atcgcctaca tccccatcca gatcggcaac ctcaccaacc tggagcgcct ctacctgaac cgcaacaaga tcgagaagat ccccacccag ctcttctact gccgcaagct gcgctacctg gacctcagcc acaacaacct gaccttcctc cctgccgaca tcggcctcct gcagaacctc cagaacctag ccatcacggc caaccggatc gagacgctcc ctccggagct cttccagtgc cggaagctgc gggccctgca cctgggcaac aacgtgctgc agtcactgcc ctccagggtg ggcgagctga ccaacctgac gcagatcgag ctgcggggca accggctgga gtgcctgcct gtggagctgg gcgagtgccc actgctcaag cgcagcggct tggtggtgga ggaggacctg ttcaacacac tgccacccga ggtgaaggag cggctgtgga gggctgacaa ggagcaggcc Protein function: Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731, PubMed:29769723). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24725410, PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E), channel characteristics depend on the precise subunit composition (PubMed:24790029, PubMed:26824658, PubMed:28193731). Can form functional channels by itself (in vitro) (PubMed:26824658). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (PubMed:14660746). Also required for T-cell development. May play a role in lysosome homeostasis (PubMed:31270356). [The UniProt Consortium]