CYP51A1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 1530 Sequence: atggcggcgg cggctgggat gctgctgctg ggcttgctgc aggcgggtgg gtcggtgctg ggccaggcga tggagaaggt gacaggcggc aacctcttgt ccatgctgct gatcgcctgc gccttcaccc tcagcctggt ctacctgatc cgtctggccg ccggccacct ggtccagctg cccgcagggg tgaaaagtcc tccatacatt ttctccccaa ttccattcct tgggcatgcc atagcatttg ggaaaagtcc aattgaattt ctagaaaatg catatgagaa gtatggacct gtatttagtt ttaccatggt aggcaagaca tttacttacc ttctggggag tgatgctgct gcactgcttt ttaatagtaa aaatgaagac ctgaatgcag aagatgtcta cagtcgcctg acaacacctg tgtttgggaa gggagttgca tacgatgtgc ctaatccagt tttcttggag cagaagaaaa tgttaaaaag tggccttaac atagcccact ttaaacagca tgtttctata attgaaaaag aaacaaagga atactttgag agttggggag aaagtggaga aaaaaatgtg tttgaagctc tttctgagct cataatttta acagctagcc attgtttgca tggaaaggaa atcagaagtc aactcaatga aaaggtagca cagctgtatg cagatttgga tggaggtttc agccatgcag cctggctctt accaggttgg ctgcctttgc ctagtttcag acgcagggac agagctcatc gggaaatcaa ggatattttc tataaggcaa tccagaaacg cagacagtct caagaaaaaa ttgatgacat tctccaaact ttactagatg ctacatacaa ggatgggcgt cctttgactg atgatgaagt agcagggatg cttattggat tactcttggc agggcagcat acatcctcaa ctactagtgc ttggatgggc ttctttttgg ccagagacaa aacacttcaa aaaaaatgtt atttagaaca gaaaacagtc tgtggagaga atctgcctcc tttaacttat gaccagctca aggatctaaa tttacttgat cgctgtataa aagaaacatt aagacttaga cctcctgtaa tgatcatgat gagaatggcc agaactcctc agactgtggc agggtatacc attcctccag gacatcaggt gtgtgtttct cccactgtca atcaaagact taaagactca tgggtagaac gcctggactt taatcctgat cgctacttac aggataaccc agcatcaggg gaaaagtttg cctatgtgcc atttggagct gggcgtcatc gttgtattgg ggaaaatttt gcctatgttc aaattaagac aatttggtcc actatgcttc gtttatatga atttgatctc attgatggat actttcccac tgtgaattat acaactatga ttcacacccc tgaaaaccca gttatccgtt acaaacgaag Protein function: A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25- dihydrolanosterol likely through sequential oxidative conversion of 14- alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid (PubMed:20149798, PubMed:8619637). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR, NADPH- ferrihemoprotein reductase) (PubMed:20149798, PubMed:8619637). [The UniProt Consortium]