AUH (Vector pUC, Accession No. BC020722)

Length: 933 Sequence: atggcggccg cggtggcggc ggcacctggg gccttgggat ccctgcatgc tggcggcgcc cgcctggtgg ccgcttgcag tgcgtggctc tgcccggggt tgaggctgcc cggctcgttg gcaggccggc gagcgggccc ggcgatctgg gcccagggct gggtacctgc ggccgggggt cccgccccga aaaggggcta cagctctgag atgaagacgg aggacgagct gcgggtgcgg cacctggagg aggagaaccg aggaattgtg gtgcttggaa taaacagagc ttatggcaaa aattcactca gtaaaaatct tataaaaatg ctatcaaaag ctgtggatgc tttgaaatct gataagaaag tacggaccat aataatcagg agtgaagtcc cagggatatt ctgtgctgct aatcttccag taccaacaat tgcagcaata gatggactcg ctttaggtgg tggtcttgaa ctggctttag cctgtgatat acgagtagca gcttcctctg caaaaatggg cctggttgaa acaaaattgg cgattattcc tggtggaggg gggacacagc gattgccacg cgccattgga atgtccctgg ccaaggagct catattctct gcgcgagtcc tcgatggcaa agaagccaaa gcagtgggct taatcagcca cgttctggaa cagaaccagg agggagacgc ggcctacagg aaggccttgg acctggcgag agagttttta cctcagggac ctgttgcaat gagagtggca aaattagcaa ttaatcaagg gatggaggtc gatttagtaa cagggttagc catagaagaa gcttgttatg ctcagaccat tccaacaaaa gacagacttg aaggtcttct tgcttttaaa gagaaaaggc cccctcgcta taaaggagaa Protein function: Catalyzes the conversion of 3-methylglutaconyl-CoA to 3- hydroxy-3-methylglutaryl-CoA (PubMed:11738050, PubMed:12434311, PubMed:12655555). Also has itaconyl-CoA hydratase activity by converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed:29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite (PubMed:29056341). Has very low enoyl-CoA hydratase activity (PubMed:7892223). Was originally identified as RNA-binding protein that binds in vitro to clustered 5'-AUUUA-3' motifs (PubMed:7892223). [The UniProt Consortium]