Y-27632-d4 (hydrochloride hydrate)

Y-27632-d4 is intended for use as an internal standard for the quantification of Y-27632 (Cay-10005583) by GC- or LC-MS. Y-27632 is an inhibitor of Rho-associated kinase 1 (ROCK1) and ROCK2 (Kis = 0. and 0. µM, respectively). It is selective for ROCK1 and ROCK2 over citron Rho-interacting kinase (CRIK), serine/threonine protein kinase N (PKN), PKCalpha, and myosin light-chain kinase (MLCK, Kis = 1. 1. 6, and 14 µM, respectively) but does inhibit PKA (Ki = 0. µM). Y-27632 inhibits cytokinesis in HeLa cervical cancer cells in a concentration-dependent manner. It increases neurite length in primary rat Schwann cells when used at a concentration of 10 µM. Y-27632 (10 µM) inhibits dissociation-induced apoptosis and increases colony formation in primary human embryonic stem cells (ESCs).. Solulibility: Methanol: slightly soluble: Water: slightly soluble.