Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an... read more »
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Dyes and Labeling Reagents

This category comprises chemicals for permanent and temporary labeling of organelles, proteins, and other molecules. For example, this includes the covalent linkage of a target protein, such as an antibody, with a fluorescent dye/fluorophore, allowing detection of the target protein in microscopic experiments. For the purpose of detection, proteins can also be coupled with reporter enzymes (e.g. HRP) or radioactive isotopes. To facilitate the purification of a target protein, biotinylation followed by streptavidine affinity binding has proven to be effective.

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6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4*
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS...

Item number: ABD-419

Tetramethylrhodamine-6-maleimide is the other purified single isomer of tetramethylrhodamine-5 (and 6)-maleimide mixed isomers. Compared to the 5-isomer, tetramethylrhodamine-6-maleimide is preferably used for thiol modifications of nucleotides and nucleic acids.
Application: Conjugation
291.00€ *
Review
RatioWorks(TM) Cal-520L(R)/Cy5-Dextran Conjugate *MW 10,000*
RatioWorks(TM) Cal-520L(R)/Cy5-Dextran Conjugate *MW 10,000*

Item number: ABD-20603

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and...
Application: IF, FC
728.00€ *
Review
N-Sp-DMAE, NHS ester
N-Sp-DMAE, NHS ester

Item number: ABD-26010

The acridinium NHS ester can be used to label proteins and nucleic acids. The covalently bound acridinium NHS esters generate chemiluminescence in the presence of hydrogen peroxide. Exposure of an acridinium ester label to an alkaline hydrogen peroxide solution triggers a flash of light. Acridinium-labeled proteins...
Application: Protein & nucleic acid chemiluminescenc labeling
291.00€ *
Review
mFluor(TM) Blue 580 SE
mFluor(TM) Blue 580 SE

Item number: ABD-1178

Molecular weight: 832.98. Advances in spectral flow cytometers have expanded applications and capabilities beyond conventional flow cytometry. Now with spectral flow cytometry analysis, researchers and scientists can investigate an increasing number of molecules of interest. However, the potential of spectral flow...
Application: Conjugation, antibody labeling, protein labeling, spectral fluorescence FC
MW: 832.98 D
416.00€ *
Review
iFluor(TM) 488 succinimidyl ester
iFluor(TM) 488 succinimidyl ester

Item number: ABD-71023

Molecular weight: 945.07 Excitation (nm): 491, Emission (nm): 516, Extinction coefficient (cm-1 M-1): 75000, Quantum yield: 0.91, Correction factor (280 nm): 0.11. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC,...
Keywords: SE
Application: Amine-reactive conjugation
MW: 945.07 D
From 108.00€ *
Review
iFluor(TM) 647 succinimidyl ester
iFluor(TM) 647 succinimidyl ester

Item number: ABD-71031

Molecular weight: 1274.66 Excitation (nm): 656, Emission (nm): 670, Extinction coefficient (cm-1 M-1): 250000, Quantum yield: 0.25, Correction factor (280 nm): 0.03. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal...
Keywords: SE
Application: Amine-reactive conjugation
MW: 1274.66 D
From 108.00€ *
Review
iFluor(TM) 430 succinimidyl ester
iFluor(TM) 430 succinimidyl ester

Item number: ABD-71052

Molecular weight: 688.79 Excitation (nm): 433, Emission (nm): 498, Extinction coefficient (cm-1 M-1): 40000, Quantum yield: 0.78, Correction factor (280 nm): 0.3. AAT Bioquest's iFluor(TM) dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal...
Keywords: SE
Application: Amine-reactive conjugation
MW: 688.79 D
From 108.00€ *
Review
iFluor(TM) 820 succinimidyl ester
iFluor(TM) 820 succinimidyl ester

Item number: ABD-71399

Molecular weight: 1585.92 Excitation (nm): 822, Emission (nm): 850, Extinction coefficient (cm-1 M-1): 250000, Correction factor (280 nm): 0.16. In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon...
Keywords: SE
Application: Amine-reactive conjugation
MW: 1585.92 D
From 108.00€ *
Review
iFluor(TM) 860 succinimidyl ester
iFluor(TM) 860 succinimidyl ester

Item number: ABD-71409

Molecular weight: 1571.9 Excitation (nm): 853, Emission (nm): 878, Extinction coefficient (cm-1 M-1): 250000, Correction factor (280 nm): 0.14. In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation...
Keywords: SE
Application: Amine-reactive conjugation
MW: 1571.9 D
From 108.00€ *
Review
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*
Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*

Item number: ABD-17026

Correction Factor (260 nm) 0.02. Correction Factor (280 nm) 0.03. Correction Factor (482 nm) 0.009. Correction Factor (565 nm) 0.09. Extinction coefficient (cm^-1 M^-1): 250000 (ethanol). Excitation (nm): 651. Emission (nm): 670. Quantum yield 0.27 (PBS), 0.4 (ethanol). The dye-modified deoxyuridine 5'-triphosphates...
Application: DNA labeling, FISH
MW: 1176.04 D
From 137.00€ *
Review
Cyanine 5-dATP Cy5-dATP
Cyanine 5-dATP Cy5-dATP

Item number: ABD-17038

Molecular weight: 1182.05. Excitation (nm): 651. Emission (nm): 670. Extinction coefficient (cm-1 M-1): 250000. Quantum yield: 0.27. Correction factor (280 nm): 0.03. The dye-modified deoxyadenosine 5'-triphosphates are widely used for a variety of non-radioactive DNA labeling reactions including nick translation,...
Application: DNA labeling, FISH
MW: 1182.05 D
137.00€ *
Review
PE-iFluor(TM) 594 Tandem
PE-iFluor(TM) 594 Tandem

Item number: ABD-2600

Excitation (nm): 566. Emission (nm): 606. Absorbance (nm): 566. Tandem dyes are a unique class of fluorescent molecules that consist of two different covalently linked fluorophores, a donor (e.g. PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Texas Red, Cy5, Cy7, iFluor(TM) 594 or iFluor(TM)...
Application: FRET, PE-Texas Red tandem replacement
291.00€ *
Review
239 from 269 pages