Cyanine-5- dUTP [Cy5-dUTP] *1 mM in TE Buffer (pH 7.5)*

Correction Factor (260 nm) 0.02. Correction Factor (280 nm) 0.03. Correction Factor (482 nm) 0.009. Correction Factor (565 nm) 0.09. Extinction coefficient (cm^-1 M^-1): 250000 (ethanol). Excitation (nm): 651. Emission (nm): 670. Quantum yield 0.27 (PBS), 0.4 (ethanol). The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This Cy5-dUTP conjugate can be used as a red fluorescence color with SpectrumFRed(TM) filter set (SpectrumFRed(TM) is the trademark of Vysis). It is functionally equivalent to Cyanine 5-dUTP (Perkin Elmer's NEL579001EA).