ZL0420

ZL0420 is an inhibitor of bromodomain 1 (BD1) and BD2 in bromodomain-containing protein 4 (BRD4, IC50s = 27 and 32 nM, respectively). It is selective for BD1 and BD2 in BRD4 over BD1 in BRD2, BRD3, and BRDT (IC50s = 803, 2,275, and 3,183 nM, respectively) and BD2 (IC50s = 1,736, 2,193, and 2,781 nM, respectively), as well as the bromodomain in CBP (IC50 = >10,000 nM). ZL0420 inhibits the expression of interferon-stimulated gene 54 (ISG54), ISG56, IL-8, and growth-regulated protein beta (Grobeta) induced by the toll-like receptor 3 (TLR3) agonist poly(I:C) by approximately 95% in human small airway epithelial cells. It also inhibits neutrophil infiltration around small- and medium-sized airways and reduces cytokine expression in lung tissue in a poly(I:C)-induced mouse model of acute airway inflammation when administered at a dose of 10 mg/kg.Formal Name: 6-[2-(2-amino-4-hydroxy-5-methylphenyl)diazenyl]-3,4-dihydro-2(1H)-quinolinone. CAS Number: 2229039-45-4. Molecular Formula: C16H16N4O2. Formula Weight: 296.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:9): 0.1 mg/ml. lambdamax: 269, 349, 448 nm. SMILES: OC1=C(C)C=C(/N=N/C2=CC(CCC(N3)=O)=C3C=C2)C(N)=C1. InChi Code: InChI=1S/C16H16N4O2/c1-9-6-14(12(17)8-15(9)21)20-19-11-3-4-13-10(7-11)2-5-16(22)18-13/h3-4,6-8,21H,2,5,17H2,1H3,(H,18,22)/b20-19+. InChi Key: ANMQADUROYWADA-FMQUCBEESA-N.