TNP-ATP (sodium salt)

TNP-ATP is a derivative of ATP and an antagonist of the purinergic P2Y1, P2X3, and P2X2/3 receptors (IC50s = 6, 0.9, and 7 nM, respectively, in HEK293 cells expressing the human receptors). It is selective for these receptors over the purinergic P2X2, P2X4, and P2X7 receptors (IC50s = 2, 15.2, and >30 µM, respectively, in HEK293 cells expressing the human receptors). TNP-ATP decreases acetic acid-induced calcium flux in 1321N1 cells expressing the P2X3 and P2X2/3 receptors (IC50s = 100 and 62 nM, respectively). It reduces acetic acid-induced writhing in a mouse model of visceral pain (ED50 = 6.35 µmol/kg). It displays an emission maximum of 547 nm upon excitation at 403 nm, and its relative fluorescence increases four-fold when bound to insulin-degrading enzyme (IDE) and emission shifts to 538 nm.Formal Name: ((3a'R,4'R,6'R,6a'R)-4'-(6-amino-9H-purin-9-yl)-2,4,6-trinitro-3a',4',6',6a'-tetrahydrospiro[cyclohexane-1,2'-furo[3,4-d][1,3]dioxole]-2,5-dien-6'-yl)methyl triphosphate, tetrasodium salt. Molecular Formula: C16H13N8O19P3 . 4Na. Formula Weight: 806.2. Purity: >95%. Emission: 547 nm. Excitation: 403 nm. Formulation: (Request formulation change), A solution in water. Solubility: Water: Soluble. SMILES: O=P(OP([O-])([O-])=O)([O-])OP(OC[C@H]1O[C@@H](N2C=NC3=C(N)N=CN=C32)[C@@]4([H])[C@]1([H])OC5(C([N+]([O-])=O)=CC([N+]([O-])=O)C=C5[N+]([O-])=O)O4)([O-])=O.[Na+].[Na+].[Na+].[Na+]. InChi Code: InChI=1S/C16H17N8O19P3.4Na/c17-13-10-14(19-4-18-13)21(5-20-10)15-12-11(7(39-15)3-38-45(34,35)43-46(36,37)42-44(31,32)33)40-16(41-12)8(23(27)28)1-6(22(25)26)2-9(16)24(29)30,,,,/h1-2,4-7,11-12,15H,3H2,(H,34,35)(H,36,37)(H2,17,18,19)(H2,31,32,33),,,,/q,4*+1/p-4/t6?,7-,11-,12-,15-,16?,,,,/m1..../s1. InChi Key: KCHIITPKWYBGJE-YXXRFPCHSA-J.