S-trityl-L-Cysteine

S-trityl-L-Cysteine is a non-natural amino acid and an inhibitor of Eg5, also known as KSP and Kif11, a mitotic kinesin necessary for mitotic spindle formation. S-trityl-L-Cysteine inhibits the ATPase activity of Eg5 in basal and microtubule-stimulated states (IC50s = 1,000 and 140 nM, respectively). It is selective for Eg5 over nine other human kinesins in an enzyme-coupled assay. It reversibly inhibits Eg5-driven microtubule sliding velocity with an IC50 value of 500 nM using X. laevis recombinant Eg5. It induces cell cycle arrest in HeLa cells (IC50 = 700 nM), reversibly halting the cell cycle in the mitotic phase by inhibiting the separation of duplicated chromosomes and preventing bipolar spindle formation. S-trityl-L-Cysteine inhibits the growth of cancer cells in vitro when tested against the National Cancer Institute (NCI) 60 human cancer cell line panel (average GI50 = 1.3 µM) and in mouse xenograft models.Formal Name: S-(triphenylmethyl)-L-cysteine. CAS Number: 2799-07-7. Synonyms: NSC 83265. Molecular Formula: C22H21NO2S. Formula Weight: 363.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: slightly soluble, Methanol: 1 mg/ml. SMILES: N[C@H](C(O)=O)CSC(C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3. InChi Code: InChI=1S/C22H21NO2S/c23-20(21(24)25)16-26-22(17-10-4-1-5-11-17,18-12-6-2-7-13-18)19-14-8-3-9-15-19/h1-15,20H,16,23H2,(H,24,25)/t20-/m0/s1. InChi Key: DLMYFMLKORXJPO-FQEVSTJZSA-N.