RI-1

RI-1 is an irreversible inhibitor of RAD51 (IC50s = 5-30 µM), a protein that is central to homologous recombination during DNA repair and is overexpressed in a wide range of human cancer cell types. RI-1 selectively inhibits homologous recombination without inhibiting single strand annealing. It binds directly to the C319 thiol of RAD51 and inhibits oligomerization of RAD51 filaments. In vitro, RI-1 sensitizes cancer cells to chemotherapeutics that generate interstrand DNA crosslinks, including HEK293 cells co-treated with mitomycin C (LD50 = 16.62 µM).Formal Name: 3-chloro-1-(3,4-dichlorophenyl)-4-(4-morpholinyl)-1H-pyrrole-2,5-dione. CAS Number: 415713-60-9. Molecular Formula: C14H11Cl3N2O3. Formula Weight: 361.6. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 13 mg/ml, DMF:PBS(pH7.2) (1:2): 0.3 mg/ml, DMSO: 2 mg/ml. lambdamax: 255, 397 nm. SMILES: ClC1=C(Cl)C=C(N2C(C(Cl)=C(N3CCOCC3)C2=O)=O)C=C1. InChi Code: InChI=1S/C14H11Cl3N2O3/c15-9-2-1-8(7-10(9)16)19-13(20)11(17)12(14(19)21)18-3-5-22-6-4-18/h1-2,7H,3-6H2. InChi Key: MWSUIZKGNWELRF-UHFFFAOYSA-N.