N6F11

N6F11 is an inducer of ferroptosis. It increases the thermal stability of the ubiquitin ligase TRIM25 in a cellular thermal shift assay (CETSA) using PANC-1 pancreatic cancer cells when used at a concentration of 10 µM and enhances TRIM25-dependent ubiquitination of glutathione peroxidase 4 (GPX4) in a cell-free assay at 0.1 and 1 µM. N6F11 (5 µM) induces lipid peroxidation and ferroptosis, as well as degradation of GPX4, an effect that can be reversed by the proteasome inhibitors carfilzomib (Cay-17554) or bortezomib (Cay-10008822), in PANC-1 cells. It selectively induces cytotoxicity in HT-1080, PANC-1, MiaPaCa-2, and Calu-1 cancer cells over neutrophils, dendritic cells, natural killer (NK) cells, and CD8+ T cells. N6F11 (10 mg/kg) decreases tumor volume in a KPC murine pancreatic ductal adenocarcinoma (PDAC) model, which can be inhibited by the ferroptosis inhibitor liproxstatin-1 (Cay-17730) or an anti-CD8 antibody.Formal Name: 2-(chloromethyl)-6-(4-chlorophenyl)-thieno[3,2-d]pyrimidin-4(3H)-one. CAS Number: 851398-76-0. Molecular Formula: C13H10Cl2N2OS. Formula Weight: 313.2. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: Acetonitrile: Slightly soluble, Chloroform: Soluble. SMILES: ClC1=CC=C(C=C1)C2=CC3C(C(NC(CCl)=N3)=O)S2. InChi Code: InChI=1S/C13H10Cl2N2OS/c14-6-11-16-9-5-10(19-12(9)13(18)17-11)7-1-3-8(15)4-2-7/h1-5,9,12H,6H2,(H,16,17,18). InChi Key: WVDCOGFCNRPLDW-UHFFFAOYSA-N.