KRIBB3

KRIBB3 is an Hsp27 and microtubule inhibitor that inhibits migration and invasion of MDA-MB-231 cells in vitro in an Hsp27-dependent manner. It inhibits protein kinase C (PKC) phosphorylation of Hsp27 but not phospholipase Cbeta3, indicating KRIBB3 interaction with Hsp27 is preventing its phosphorylation. KRIBB3 (1-100 µM) inhibits growth of HCT116, HCT15, and MDA-MB-231 cells in an Hsp27-independent and concentration-dependent manner. It induces HCT116 cell cycle arrest in the G2/M phase via formation of the Mad2/p55CDC inhibitory complex and Bax activation of apoptosis. KRIBB3 also inhibits microtubule polymerization in HCT116 cells. In vivo, KRIBB3 (50-100 mg/kg) reduces tumor volume in an HCT116 mouse xenograft model.Formal Name: 4-ethyl-5-methoxy-2-[4-(4-methoxyphenyl)-5-isoxazolyl]-phenol. CAS Number: 129414-88-6. Molecular Formula: C19H19NO4. Formula Weight: 325.4. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMSO: 25 mM. SMILES: OC1=CC(OC)=C(CC)C=C1C2=C(C3=CC=C(OC)C=C3)C=NO2. InChi Code: InChI=1S/C19H19NO4/c1-4-12-9-15(17(21)10-18(12)23-3)19-16(11-20-24-19)13-5-7-14(22-2)8-6-13/h5-11,21H,4H2,1-3H3. InChi Key: BNBHIGLOMVMJDB-UHFFFAOYSA-N.