CPYPP

CPYPP is an inhibitor of the Rac activators dedicator of cytokinesis 2 (DOCK2, IC50 = 22.8 µM for DOCK2 guanine nucleotide exchange factor (GEF) activity), DOCK5, and DOCK180. It is selective for these DOCK-A subfamily proteins over the DOCK-D subfamily member DOCK9. CPYPP (100 µM) prevents chemokine-induced Rac activation in T and B cells. It also inhibits chemokine-induced migration of T and B cells. CPYPP (5 mg/animal, i.p.) reduces migration of T cells to peripheral lymph nodes in mice following adoptive transfer of spleen cells from knock-in mice expressing GFP-tagged DOCK2. It also reduces myeloperoxidase (MPO) activity in lung tissue, LPS-induced cytokine release in serum, and the severity of lung injury in a mouse model of endotoxemia-induced acute lung injury when administered at a dose of 250 mg/kg.Formal Name: 4-[3-(2-chlorophenyl)-2-propen-1-ylidene]-1-phenyl-3,5-pyrazolidinedione. CAS Number: 310460-39-0. Molecular Formula: C18H13ClN2O2. Formula Weight: 324.8. Purity: >98% (mixture of isomers). Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 50 mM. lambdamax: 247, 362 nm. SMILES: O=C(N(C1=CC=CC=C1)NC/2=O)C2=C/C=C/C3=C(Cl)C=CC=C3. InChi Code: InChI=1S/C18H13ClN2O2/c19-16-12-5-4-7-13(16)8-6-11-15-17(22)20-21(18(15)23)14-9-2-1-3-10-14/h1-12H,(H,20,22)/b8-6+,15-11-. InChi Key: VVZJFICTTKPNCK-XWDZFIQBSA-N.