Clozapine N-oxide (hydrochloride)

Clozapine N-oxide is a major metabolite of clozapine (Cay-12059) and an activator of designer receptors exclusively activated by designer drugs (DREADDs) derived from human muscarinic acetylcholine receptors (EC50s = 16. 323, 17. 18. and 18. nM for PASMCs expressing hM1-5D receptors, respectively). It prevents action potential firing in cultured hippocampal neurons transiently expressing hM4D receptors. Clozapine N-oxide increases glutamate in the nucleus accumbens core and inhibits cue-induced reinstatement of cocaine-seeking behavior in rats transfected with hM3Dq DREADD receptors. Clozapine N-oxide is a major metabolite of clozapine (Cay-12059) and an activator of designer receptors exclusively activated by designer drugs (DREADDs) derived from human muscarinic acetylcholine receptors (EC50s = 16.7, 323, 17.4, 18.3, and 18.7 nM for PASMCs expressing hM1-5D receptors, respectively). It prevents action potential firing in cultured hippocampal neurons transiently expressing hM4D receptors. Clozapine N-oxide increases glutamate in the nucleus accumbens core and inhibits cue-induced reinstatement of cocaine-seeking behavior in rats transfected with hM3Dq DREADD receptors.Formal Name: 8-chloro-11-(4-methyl-4-oxido-1-piperazinyl)-5H-dibenzo[b,e][1,4]diazepine, dihydrochloride. CAS Number: 2250025-93-3. Synonyms: CNO. Molecular Formula: C18H19ClN4O . 2HCl. Formula Weight: 415.7. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMSO: 25 mg/ml, Ethanol: 25 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 213, 226 nm. SMILES: C[N]1(CCN(C2=NC(C=C(Cl)C=C3)=C3NC4=CC=CC=C24)CC1)=O.Cl.Cl. InChi Code: InChI=1S/C18H19ClN4O.2ClH/c1-23(24)10-8-22(9-11-23)18-14-4-2-3-5-15(14)20-16-7-6-13(19)12-17(16)21-18,,/h2-7,12,20H,8-11H2,1H3,2*1H. InChi Key: MBRGKRXDVKTUPT-UHFFFAOYSA-N. InChI: InChI=1S/C18H19ClN4O.2ClH/c1-23(24)10-8-22(9-11-23)18-14-4-2-3-5-15(14)20-16-7-6-13(19)12-17(16)21-18,,/h2-7,12,20H,8-11H2,1H3,2*1H InChIKey: MBRGKRXDVKTUPT-UHFFFAOYSA-N SMILES: C[N+]1(CCN(C2=NC(C=C(Cl)C=C3)=C3NC4=CC=CC=C24)CC1)[O-].Cl.Cl