C527

C527 is an inhibitor of USP1/UAF complex deubiquitinase activity (IC50 = 0.88 µM). It also inhibits USP5, UCL-H3, and USP12/46 but not UCL-H1 activity (IC50s = 1.65, 2.18, 5.97, and >10 µM, respectively). C527 increases ubiquinated Fanconi anemia complementation group D2 (FANCD2-Ub) and FANCI-Ub levels in a concentration-dependent manner. It inhibits homologous recombination repair, cell proliferation, and sensitizes HeLa cells to the DNA crosslinker mitomycin C (Cay-11435). C527 also inhibits proliferation of PC3 and LNCap prostate cancer cells (IC50s = 0.24 and 0.03 µM, respectively, after 72 hours).Formal Name: 2-(4-fluorophenyl)-naphth[2,3-d]oxazole-4,9-dione. CAS Number: 192718-06-2. Molecular Formula: C17H8FNO3. Formula Weight: 293.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 0.33 mg/mL, DMSO: 0.16 mg/mL. lambdamax: 277 nm. SMILES: O=C(C(OC(C1=CC=C(F)C=C1)=N2)=C2C3=O)C4=C3C=CC=C4. InChi Code: InChI=1S/C17H8FNO3/c18-10-7-5-9(6-8-10)17-19-13-14(20)11-3-1-2-4-12(11)15(21)16(13)22-17/h1-8H. InChi Key: ULJDFEYQOPCCPM-UHFFFAOYSA-N.