Ac-YVAD-AFC

Ac-YVAD-AFC is a substrate whose amino acids YVAD have been shown to be a preferred cleavage site for caspase-1 and -4. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.Formal Name: N-acetyl-L-tyrosyl-L-valyl-L-alanyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine. CAS Number: 219137-85-6. Synonyms: Caspase-1 Substrate VI (Fluorogenic), Ac-Tyr-Val-Ala-Asp-7-amino-4-Trifluoromethlcoumarin. Molecular Formula: C33H36F3N5O10. Formula Weight: 719.7. Purity: >95%. Emission: 505 nm. Excitation: 400 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 10 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 339 nm. SMILES: O=C([C@H](C)NC([C@H](C(C)C)NC([C@H](CC1=CC=C(O)C=C1)NC(C)=O)=O)=O)N[C@H](C(NC2=CC=C3C(C(F)(F)F)=CC(OC3=C2)=O)=O)CC(O)=O. InChi Code: InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(. InChi Key: GWKJISJKNKIJNP-DEQDFGQZSA-N.