Ac-IEPD-pNA (trifluoroacetate salt)

Ac-IEPD-pNA is a colorimetric substrate for granzyme B and caspase-8. Granzyme B and caspase-8 preferentially bind to and cleave the Ile-Glu-Pro-Asp (IEPD) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of granzyme B and caspase-8 activity.Formal Name: N-acetyl-L-isoleucyl-L-alpha-glutamyl-L-prolyl-N-(4-nitrophenyl)-L-alpha-asparagine, trifluoroacetate salt. Synonyms: Ac-Ile-Glu-Pro-Asp-p-nitroanilide, Caspase-8 Chromogenic Substrate, Granzyme B Substrate VIII. Molecular Formula: C28H38N6O11 . XCF3COOH. Formula Weight: 634.6. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: DMSO: 1 mg/ml. SMILES: O=C(C)N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@@H](CC(O)=O)C(NC2=CC=C([N+]([O-])=O)C=C2)=O)=O)=O)CCC(O)=O)=O)[C@@H](C)CC.FC(F)(C(O)=O)F. InChi Code: InChI=1S/C28H38N6O11.C2HF3O2/c1-4-15(2)24(29-16(3)35)27(42)31-19(11-12-22(36)37)28(43)33-13-5-6-21(33)26(41)32-20(14-23(38)39)25(40)30-17-7-9-18(10-8-17)34(44)45,3-2(4,5)1(6)7/h7-10,15,19-21,24H,4-6,11-14H2,1-3H3,(H,29,35)(H,30,40)(H,31,42)(H,32,41)(H,36,37)(H,38,39),(H,6,7)/t15-,19-,20-,21-,24-,/m0./s1. InChi Key: WIDZNAURMGQRCO-QIWSZQIESA-N.