5-FAM-YVAD-FMK

Molecular weight: 854.84. Excitation (nm): 493. Emission (nm): 517. Extinction coefficient (cm-1 M-1): 83000. Correction factor (280 nm): 0.178. Correction factor (260 nm): 0.32. FAM-YVAD-FMK, i.e., 5-carboxyfluorescein-Tyr-Val-Ala-Asp-fluoromethylketone, binds irreversibly to active caspase 1 in stimulated cells. The fluorescent intensity of the FAM-YVAD-FMK signal is proportional to the amount of active caspase 1 and can be easily detected at by fluorescence microscopy, flow cytometer, or fluorescent microplate reader.