Androgen Receptor Luciferase Reporter Lentivirus

Androgen Receptor Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase reporter driven by multiple copies of androgen response element (ARE) located upstream of the minimal TATA promoter. The lentiviruses also contain a puromycin selection marker (Figure 1). After transduction, the cellular androgen receptor signaling pathway can be monitored by measuring the luciferase activity. This product has been validated by testing the response to 5alpha-Dihydrotestosterone (5-DHT) of the transduced prostate cancer cell lines 22Rv1 and CWR-R1ca, but not LNcaP.