Amylin Receptor 3 (AMY3R)/CRE Luciferase Reporter HEK293 Cell Line

The Amylin Receptor 3 (AMY3R)/CRE Luciferase Reporter HEK293 Cell Line is a HEK293 cell line engineered to express CALCR (calcitonin receptor, NM_001164737.3) and RAMP3 (receptor activity modifying protein 3, NM_005856.3), delivered into CRE/CREB Luciferase Reporter HEK293 Cell Line (cAMP/PKA Signaling Pathway) (#60515), which expresses a firefly luciferase reporter driven by a multimerized cAMP response element (CRE). Activation of the receptor in these cells can be monitored by measuring luciferase activity. This cell line was validated in dose-response assays using the peptide agonists Amylin, Calcitonin, Pramlintide, Cagrilintide, and Eloralintide. Purchase of this cell line is for research purposes only, commercial use requires a separate license. Amylin, also known as islet amyloid polypeptide or IAPP, is a peptide hormone produced by pancreatic beta-cells and secreted alongside insulin, downstream of nutrient sensing. Circulating amylin signals through the amylin family of receptors which are comprised of two subunits, the shared G-protein calcitonin receptor (CALCR) and one of three receptor activity-modifying protein subunits (RAMPs 1-3) to generate AMY1R, AMY2R and AMY3R, respectively. The receptors signal downstream through the second messenger cAMP. All three subtypes of the amylin receptor are expressed in brain regions that control feeding and satiety. Amylin action through the AMYRs plays a role in satiety and control of blood glucose levels. Amylin agonists and dual amylin/ calcitonin receptor agonists are currently under development for the treatment of both obesity and type-2 diabetes (T2D).