Normal Human Peripheral Blood Mononuclear Cells, Frozen

Cryopreserved vial (100 x 10^6, 30 x 10^6, 10 x 10^6 or 2 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis /apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.