Rat MC4R (Melanocortin receptor 4) ELISA (Small Sample Volume)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MC4R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MC4R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MC4R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MC4R in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: Hormone receptor that acts as a key component of the leptin-melanocortin pathway at the intersection of homeostatic maintenance of energetic state. Plays a role in regulating food intake: activation by a stimulating hormone such as anorexigenic alpha-melanocyte stimulating hormone (alpha-MSH) inhibits appetite, whereas binding to a natural antagonist like Agouti-related protein/AGRP promotes appetite. G-protein-coupled receptor that activates conventional Galphas signaling leading to induction of anorexogenic signaling in the hypothalamus to result in negative energy balance (By similarity). Regulates the firing activity of neurons from the hypothalamus by alpha-MSH and AGRP independently of Galphas signaling by ligand-induced coupling of closure of inwardly rectifying potassium channel KCNJ13 (By similarity). In intestinal epithelial cells, plays a role in the inhibition of hepatic glucose production via nesfatin-1/NUCB2 leading to increased cyclic adenosine monophosphate (cAMP) levels and glucagon-like peptide 1 (GLP-1) secretion in the intestinal epithelium (By similarity) [The Uniprot Consortium]