Rat GLP1R (Glucagon Like Peptide 1 Receptor) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GLP1R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GLP1R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GLP1R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GLP1R in the samples is then determined by comparing the OD of the samples to the standard curve. Protein Function: G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:1326760, PubMed:7527026, PubMed:7813606). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed:1326760, PubMed:7813606). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity) [The Uniprot Consortium]