Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) BioAssay(TM) ELISA Kit (Human)

Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) BioAssay(TM) ELISA Kit (Human)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
517540.96 96 tests - -

3 - 19 business days*

1,037.00€
 
Sample Type:|Tissue homogenates, cell lysates and other biological fluids||Intended Use:|The kit... more
Product information "Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) BioAssay(TM) ELISA Kit (Human)"
Sample Type:, Tissue homogenates, cell lysates and other biological fluids, Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIN1 in human tissue homogenates, cell lysates and other biological fluids. Sensitivity: 0.054ng/ml, Range: 0.156-10ng/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1)., No significant cross-reactivity or interference between Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12%, Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to PIN1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIN1, biotin-conjugated antibody and, enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate , Standard, 2x vial, Detection Reagent A, 1×120ul, Detection Reagent B, 1×120ul, TMB Substrate, 1×9ml, Wash Buffer (30X), 1×20ml, Standard Diluent, 1×20ml, Assay Diluent A, 1×12ml, Assay Diluent B, 1×12ml, Stop Solution, 1x6ml, Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: PIN1, EC=5.2.1.8, PPIase Pin1, Rotamase Pin1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
Supplier: United States Biological
Supplier-Nr: 517540

Properties

Application: ELISA
Species reactivity: human
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: °C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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