Naveni CD8/MHCI Atto647N

The Naveni(R) CD8/MHC-I Atto647N is an in situ proximity ligation assay kit for the detection of the interaction of CD8 and MHC-I. This in situ kit gives a high signal-to-noise ratio and has outstanding specificity. The kit is compatible with immunofluorescence co-staining for visualization of the tumor microenvironment. The kit has been validated on FFPE human tissue samples. Not recommend for mouse tissue. For research use only. Not for use in diagnostic procedures. The kit is built upon our Naveni(R) in situ proximity ligation technology, featuring two Navenibodies linked to proprietary oligo arms. This technology ensures precise and sensitive detection. The primary antibodies included in the kit bind to CD8 and MHC-I proteins, while Navenibodies then bind to their respective primary antibodies. When Navenibodies are in close proximity, a strong and distinct signal is generated, offering a specific and sensitive detection method for the interaction between CD8 and MHC-I. Included in the kit: Primary antibody targeting human CD8 (Based on clone SP239 Abcam) Primary antibody targeting human MHC-I Two Navenibodies targeting respectively primary antibody Detection fluorophore Atto647N Buffers for blocking and dilutions and all detection reagents The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions) Not included in the kit: Tris EDTA antigen retrieval buffer pH 9 Wash buffer DAPI nuclear stain Mounting medium