Mouse WISP1(WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit

Downstream regulator in the Wnt/Frizzled-signaling pathway (By similarity). Associated with cell survival. Adheres to skin and melanoma fibroblasts (By similarity). In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan (By similarity). Suppresses tumor growth in vivo Assay Type: Sandwich. Sensitivity: 21.53 pg/mL. Standard: 5000 pg/mL. Detection range: 78.13-5000 pg/mL. Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h. Research Field: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse WISP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse WISP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse WISP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse WISP1 in the samples is then determined by comparing the OD of the samples to the standard curve.