Mouse PGAM1 (Phosphoglycerate Mutase 1, Brain) ELISA Kit

Mouse PGAM1 (Phosphoglycerate Mutase 1, Brain) ELISA Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ELK-ELK7823.48 48 wells - -

10 - 14 business days*

365.00€
ELK-ELK7823.96 96 wells - -

10 - 14 business days*

522.00€
 
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate... more
Product information "Mouse PGAM1 (Phosphoglycerate Mutase 1, Brain) ELISA Kit"
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PGAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PGAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PGAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PGAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.. Detection Range: 0.32-20 ng/mL. Sensitivity: 0.125 ng/mL. Assay Type: Sandwich. Standard: 20 ng/mL. Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay duration: 3.5h
Keywords: PGAM-B, BPG-dependent PGAM 1, Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B
Supplier: ELK Biotechnology
Supplier-Nr: ELK7823

Properties

Application: ELISA
Species reactivity: mouse
Format: Solid Phase

Handling & Safety

Storage: vT
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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