Mouse NCAN(Neurocan) ELISA Kit

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan, binds to hyaluronic acid Assay Type: Sandwich. Sensitivity: 21.3 pg/mL. Standard: 5000 pg/mL. Detection range: 78.13-5000 pg/mL. Sample type: Tissue homogenates and other biological fluids.. Assay length: 3.5h. Research Field: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NCAN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NCAN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NCAN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NCAN in the samples is then determined by comparing the OD of the samples to the standard curve.