Lactate Dehydrogenase B (LDHB) BioAssay(TM) ELISA Kit (Human)

The Human Lactate Dehydrogenase B (LDHB) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LDHB in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml, Sensitivity: 0.133ng/ml, Intra-Assay CV: <10%, Inter-Assay CV: <12%, Kit Components: *026420A: Microtiter Plate, 1x48wells or 1x96 wells, Pre-coated, ready to use., *026420B: Standard, 2x1vial , 026420C: Standard Diluent, 1x10ml or 1x20ml, *026420D: Detection Reagent A (green), 1x60ul or 1x120ul , *026420E: Detection Reagent B (red), 1x60ul or 1x120ul , 026420F: Assay Diluent A, 1x6ml or 1x12ml, 026420G: Assay Diluent B, 1x6ml or 1x12ml, 026420H: TMB Substrate, 1x4.5ml or 1x9ml, 026420K: Stop Solution, 1x3ml or 1x6ml, 026420L: Wash Buffer, 30X, 1x10ml or 1x20ml , Precaution: The Stop Solution (026420K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *026420A, *026420B, *026420D and *026420E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards., 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C., 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C., 4. Aspirate and wash 3 times., 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C., 6. Aspirate and wash 5 times., 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C., 8. Add 50ul Stop Solution. Read at 450nm immediately.