Human MMP-1 ELISA Kit

Matrix metalloproteinase-1 (MMP-1), commonly known as collagenase, is a member of the matrix metalloproteinases (MMPs) family. These are zinc-dependent proteases that degrade extracellular matrix proteins. The MMPs comprise of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. MMP-1 plays an important role in the degradation of collagen in inflammatory diseases. MMP-1 (interstitial collagenase), MMP-9 (gelatinase B) and MMP-12 (macrophage elastase) are thought to be important in the development of emphysema. Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking. The standard product used in this kit is human MMP-1 with the molecular mass of 51-53 KDa. The detected MMP-1 includes zymogen and active enzyme. ScienCell's human MMP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MMP-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human MMP-1 captured in strips.