ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of... read more »
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ELISA and Immunoassays

Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique to detect the presence of an antibody or an antigen in a sample. Whereas an indirect ELISA is used to determine the amount of antibodies to a specific antigen in a sample, a Sandwich-ELISA is used to determine the concentration of the analyte by use of antibodies that bind to the analyte (and a standard). A different way of determining the analyte concentration is a competitive ELISA, often called EIA. This technique is preferred for small analytes.

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Cyclic CMP ELISA Kit
Cyclic CMP ELISA Kit

Item number: Cay502480-96

Cayman's Cyclic CMP ELISA Kit is a competitive assay that can be used for the quantification of cCMP in bacterial lysates. The assay has a range of 0.156-20 nM, an average sensitivity (80% B/B0) of 0. nM, and a lower limit of detection (LLOD) of 0. nM.
Keywords: Cyclic Cytidine 3'5'-monophosphate, cCMP
Application: ELISA
681.00€ *
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Alternative Complement Pathway, Mouse, Assay
Alternative Complement Pathway, Mouse, Assay

Item number: HYC-HIT422

The HIT422 measures the alternative pathway activity mediated via LPS. Complement deficiencies or other defects in the complement system can easily be screened by running an assay for each pathway in parallel or separately. The ELISA contains a positive control which can be used as control to ensure that the...
Keywords: AP, alternative pathway
Application: ELISA
Species reactivity: mouse
929.00€ *
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Complement Pathway, Mouse, Assay
Complement Pathway, Mouse, Assay

Item number: HYC-HIT423

The HIT423 contains three Complement Pathway Assays: - HIT420, Classical Complement Pathway, Mouse - HIT421, Lectin Complement Pathway, Mouse - HIT422, Alternative Complement Pathway, Mouse The innate immune system is important in the first defense against foreign pathogens. A major component of this response is the...
Keywords: Complement Pathway
Application: ELISA
Species reactivity: mouse
1,854.00€ *
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TCC, Rat, ELISA kit
TCC, Rat, ELISA kit

Item number: HYC-HK106-01

Protein Function: Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed:9212048). The MAC is initiated by proteolytic cleavage...
Keywords: C9, Complement component C9
Application: ELISA
Species reactivity: rat
From 858.00€ *
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MASP-1/C1-INH complex, Human, ELISA kit
MASP-1/C1-INH complex, Human, ELISA kit

Item number: HYC-HK3001-01

Protein Function: Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to...
Keywords: MASP1, Serine protease 5, Complement factor MASP-3, Ra-reactive factor serine protease p100, Mannan-binding lectin serine...
Application: ELISA
Species reactivity: human
From 929.00€ *
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FHR-4, Human, ELISA kit
FHR-4, Human, ELISA kit

Item number: HYC-HK3006-01

The FHR-4, human, ELISA kit is to be used for the in vitro quantitative determination of FHR-4 in serum and plasma samples.
Keywords: CFHR4, Complement factor H-related protein 4
Application: ELISA
Species reactivity: human
From 858.00€ *
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BPI, Human, ELISA kit
BPI, Human, ELISA kit

Item number: HYC-HK314-01

Protein Function: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria, this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial...
Keywords: BPI, CAP 57, Bactericidal permeability-increasing protein
Application: ELISA
Species reactivity: human
From 715.00€ *
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LL-37, Human, ELISA kit
LL-37, Human, ELISA kit

Item number: HYC-HK321-01

Protein Function: Antimicrobial protein that is an integral component of the innate immune system (PubMed:14978112, PubMed:16637646, PubMed:18818205, PubMed:22879591, PubMed:9736536). Binds to bacterial lipopolysaccharides (LPS) (PubMed:16637646, PubMed:18818205). Acts via neutrophil N-formyl peptide receptors to...
Keywords: CAMP, Cathelicidin antimicrobial peptide, 18 kDa cationic antimicrobial protein
Application: ELISA
Species reactivity: human
From 858.00€ *
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MBL, Human, ELISA kit
MBL, Human, ELISA kit

Item number: HYC-HK323-01

Protein Function: Calcium-dependent lectin involved in innate immune defense (PubMed:35102342). Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early...
Keywords: MBL2, MBP1, Collectin-1, Mannose-binding lectin, Mannan-binding protein, Mannose-binding protein C
Application: ELISA
Species reactivity: human
From 786.00€ *
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MASP-2, Human, ELISA kit
MASP-2, Human, ELISA kit

Item number: HYC-HK326-01

Protein Function: Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase [The Uniprot Consortium]
Keywords: MASP2, MBL-associated serine protease 2, Mannan-binding lectin serine protease 2, Mannose-binding protein-associated...
Application: ELISA
Species reactivity: human
From 929.00€ *
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SAP, Human, ELISA kit
SAP, Human, ELISA kit

Item number: HYC-HK331-01

Protein Function: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin [The Uniprot Consortium]
Keywords: APCS, Serum amyloid P-component, 9.5S alpha-1-glycoprotein
Application: ELISA
Species reactivity: human
From 715.00€ *
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Properdin, Human, ELISA kit
Properdin, Human, ELISA kit

Item number: HYC-HK334-01

Protein Function: A positive regulator of the alternate pathway (AP) of complement (PubMed:16301317, PubMed:20382442, PubMed:28264884, PubMed:9748277). It binds to and stabilizes the C3- and C5-convertase enzyme complexes (PubMed:16301317, PubMed:20382442, PubMed:28264884, PubMed:9748277). Inhibits CFI-CFH mediated...
Keywords: CFP, Properdin, Complement factor P
Application: ELISA
Species reactivity: human
From 858.00€ *
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