PARP2 Colorimetric Assay Kit

The PARP2 Colorimetric Activity Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NADdependent addition of poly(ADP-ribose) to histones. The key to the PARP2 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.