Notch1:DLL4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit

The Notch1:DLL4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding of Notch1 (neurogenic locus notch homolog protein 1) and DLL4 (delta like canonical notch ligand 4). This kit comes in a convenient 96-well format, with enough recombinant purified Notch1, biotin-labeled DLL4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled DLL4 by streptavidin-HRP. Notch1 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-DLL4, the plate is washed and Streptavidin-HRP is added. After the ELISA ECL substrate is added the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of Notch1 to DLL4.