JMJD2D Chemiluminescent Assay Kit

The JMJD2D Chemiluminescent Kit is designed to measure activity of the JMJD2D using purified enzyme complex or cell extracts containing JMJD2D. JMJD2D is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K9Me2. The JMJD2D Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD2D for 100 enzyme reactions. The key to the JMJD2D Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.