JMJD2C Homogenous Assay kit

The JMJD2C Homogenous Assay Kit is designed to measure activity of the JMJD2C using purified enzyme complex or cell extracts containing JMJD2C. JMJD2C is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. The JMJD2C Homogenous Assay Kit comes in a convenient format, with a 384-well plate, biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2C for 384 enzyme reactions. The key to the JMJD2C Homogenous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required formethyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.