CREBBP Inhibitor Screening Assay Kit

The CREBBP Inhibitor Screening Assay Kit is designed to measure the inhibition of CREBBP binding to its substrate. The CREBBP Inhibitor Screening Assay Kit comes in a convenient AlphaLISA(R) format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged CREBBP bromodomain to perform a total of 384 enzyme reactions. The key to the CREBBP Inhibitor Screening Assay Kit is a specific binding of the CREBBP bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CREBBP and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.